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assays/ techniques (RNA, DNA, and protein extraction; real-time qPCR, Western blotting, cell-function assays, ELISA, immunofluorescence staining, confocal and transmission electron microscopy, and flow
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engineering; Experience in AAV vector transduction and titration assays; Experience in the development of dual AAV vectors; Experience in flow cytometry; fluorescence microscopy, PCR, Wester Blot and ELISA
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cellular assays and validation by ELISA and qPCR, among others. Applicable legislation and regulations: Research Fellowship Holder Statute, approved by Law No. 40/2004, of 18 August, in its current wording
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behaviour, reactivity and phenotype is essential. Experience with vascular biology, inflammation or calcification is advantageous. Skills such as Western blotting, qPCR and ELISA are desirable. Analytical and