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techniques of cryo-electron microscopy (cryo-EM), small angle X-ray scattering (SAXS), single-molecule force spectroscopy (SMFS), biochemistry, mutational analysis, cell culture, activity assays, et cetera
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that are properly folded and competent for protein translation. Force spectroscopy (optical and magnetic tweezers), single-molecule fluorescence, and bulk biochemical methods will be used to investigate how
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