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RNA sequencing (RNAseq), Fluorescence-activated cell sorting (FACS), and immunoprecipitation (IP). Maintains and differentiates human induced pluripotent stem cells (iPSCs) into specific cell lineages
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that are properly folded and competent for protein translation. Force spectroscopy (optical and magnetic tweezers), single-molecule fluorescence, and bulk biochemical methods will be used to investigate how
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of the Principal Investigator, isolates extracellular vesicles (EVs) from human blood samples to quantify EV concentration and examine cargo material using techniques such as RNA sequencing (RNAseq), Fluorescence
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experience maintaining a mouse colony, as well as stereotactic surgery, brain dissection, immunofluorescence, fluorescence microscopy, and confocal microscopy. City: Piscataway State: NJ Location: Busch (RU
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, primary neuronal and glial cell culture. Must have experience maintaining a mouse colony, as well as stereotactic surgery, brain dissection, immunofluorescence, fluorescence microscopy, and confocal
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molecular biology, protein expression and protein purification, characterization of protein and protein-nucleic acids complexes using various biophysical methods (for example: EMSA, ITC, AUC, FLUORESCENCE
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, neuroscience, and/or mechanobiology are preferred. Preferred Qualifications Equipment Utilized Micropipette manipulation and patch-clamp, optical tweezers, fluorescence microscopy, cell culture, AFM, EM
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carries out experiments including cell culture (various primary cells as well as immortalized cell lines), high resolution fluorescence imaging and image analysis, biochemical as well as molecular biology