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research in this field employs high-resolution imaging, in vivo electrophysiology, genetic and genomic manipulation (e.g., CRISPR, RNAi), and computational/bioinformatic modeling to uncover how neural
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the current PhD project. This will be achieved by working with primary synovial cells obtained from joints from arthritis patients (e.g., CRISPR knock-outs and phosphoproteomics signaling), in vivo arthritis
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biology techniques including, but not limited to, PCR, protein/DNA/RNA extraction and gel electrophoresis, bacterial transformation, imaging, cloning, gene-editing (such as CRISPR), small and large scale
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routinely performed in the lab, such as FISH and CRISPR. There will also be the chance to build on techniques we have recently started using in our system, such as expansion microscopy and laser ablation
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cells, 3D vascularized cardioids, CRISPR gene editing, high-throughput CRISPRko/i/a screening, single-cell multiomics, tissue engineering, and animal models. We welcome candidates with expertise in iPSC
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: This project will investigate the nuclear function of TPL-2 kinase. Techniques: Cell culture, protein-protein interaction studies, CRISPR/Cas9 gene editing, transcriptomic analysis by QPCR and RNA-seq
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multidisciplinary approaches, including human pluripotent stem cell reprogramming and differentiation, CRISPR/Cas9–mediated genome editing, electrophysiology and patch-clamp recordings, advanced imaging techniques
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the microenvironment, with a particular focus on cancer stem-cell programs and their translation into novel treatments.? Responsibilities: Execute standardized pooled CRISPR-KO functional genomic screens in patient
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is focused on understanding the mechanisms of cardiovascular development, disease, and regeneration. For more details, please visit our lab website: https://www.nationwidechildrens.org/research/areas
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, thermodynamics of solvation shells, constant‑pH simulation methods, Markovian thermodynamics, as well as functional mechanisms of the ribosome, fatty acid synthase, CRISPR/Cas or intrinsically disordered proteins