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, primary neuronal and glial cell culture. Must have experience maintaining a mouse colony, as well as stereotactic surgery, brain dissection, immunofluorescence, fluorescence microscopy, and confocal
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that are properly folded and competent for protein translation. Force spectroscopy (optical and magnetic tweezers), single-molecule fluorescence, and bulk biochemical methods will be used to investigate how
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. More information about the lab can be obtained at https://deaconesculab.com Position Status Full Time Posting Number 25FA0742 Posting Open Date Posting Close Date Qualifications Minimum Education and
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, neuroscience, and/or mechanobiology are preferred. Preferred Qualifications Equipment Utilized Micropipette manipulation and patch-clamp, optical tweezers, fluorescence microscopy, cell culture, AFM, EM
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of the Principal Investigator, isolates extracellular vesicles (EVs) from human blood samples to quantify EV concentration and examine cargo material using techniques such as RNA sequencing (RNAseq), Fluorescence
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, confocal, and fluorescent microscopes, histopathology processor, embedding center, microtome and cryostat, ELISA plate washer, ELISA plate reader, ddPCR, qPCR, and other equipment as needed. Physical Demands
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single-cell analyses of DNA and RNA by Fluorescent in situ Hybridization (FISH), and CRISPR gene editing to induce genomic instability and study its consequences on tumor transformation and therapy
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compromising a Differential Scanning Calorimeter (DSC), Thermogravimetric Analyzer (TGA), Rheometer, and Dynamic Mechanical Analyzer (DMA), such as X-ray absorption and X-ray fluorescence instruments, and other
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carries out experiments including cell culture (various primary cells as well as immortalized cell lines), high resolution fluorescence imaging and image analysis, biochemical as well as molecular biology
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instillation or inhalation. Fluorescently labeled nanoparticles (e.g., rhodamine-tagged nanopolystyrene) with optical/fluorescent imaging. Vascular physiology and myography for vasoactivity and endothelial