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thermal shift assays. Computational analysis of genomes and of disordered protein regions. Analyze protein condensates formed through liquid-liquid phase separation. Generate knock-out P. falciparum and L
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, including genome engineering of human embryonic stem cells, fluorescent protein tagging strategies, clone validation, super-resolution microscopy, and cell-based biophysical studies of metal homeostasis
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the National Science Center project of the OPUS project of the National Science Center 2023/49/B/NZ2/00356, under the title "The interplay between nucleoid associated proteins and transcription factors
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proteins associated with human diseases. Based on structural information, hits are growth to generate complex molecules with high activity and specificity. Traditionally, this growing process driven by
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. Experience in molecular biology, bacterial culture, and protein purification techniques. Knowledge of protein analysis (SDS-PAGE) and cellular analysis (flow cytometry) is appreciated. Rigor, autonomy, and
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, transformation, transfection, transduction). DNA sequencing DNA, RNA and protein isolation techniques Gene expression analysis by Real Time PCR and semi-quantitative PCR Methods for quantitative protein
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experience in conducting research projects, including data collection and analysis Specialist expertise in or theoretical knowledge in molecular biology, biochemistry, especially protein expression
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strategies for sampling and analysis of diagnostic and therapeutic proteins in biological matrices, for example from a single drop of blood. Our goal is to bring advanced protein analysis closer to clinical
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IRF1 in inducing expression of proteins that block assembly of hepatitis C virus (DOI: 10.1371/journal.ppat.1010812), uncovering a hitherto unrecognised aspect of innate antiviral immunity. Subsequent
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experience is desirable Essential skills: Strong expertise in microbiological techniques Proven experience in the purification and analysis of protein complexes Experience in structural biology, ideally in