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characterization of the iPSCs and neuronal models via: Immunostaining, cryosectioning, and microscopy DNA/RNA extraction (q)RT-PCR Western blotting Generation of cell lines using CRISPR-Cas9 gene editing methods
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protection information on the processing of personal data as part of the application and selection process, please refer to the privacy policy on our website at https://www.senckenberg.de/en/imprint/ Please
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, harvests), multi-parameter flow and mass cytometry (CyTOF), fluorescence-activated cell sorting (FACS), multiplexed fluorescence microscopy and single-cell RNA sequencing; prepare RNA and DNA; perform PCR
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of human cell lines, human Peripheral Blood Mononuclear Cells (PMBCs), electroporation, transfection, and transduction. Basic molecular biology techniques that include PCR, DNA cloning, plasmid design and
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, confocal fluorescent imaging, research animal handling and potentially husbandry, molecular biology assays including Western blot, qRT-PCR and cloning (among others). Lab members contribute to an educational
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fundamental biological processes and to explore their potential applicability in treating neurodegenerative diseases caused by the expansion of DNA repeats. A brief project description can be found at: https
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to date, maintain equipment, routine molecular biology (e.g. PCR/qRT-PCR, Western blotting) and maintenance of databases. Additional duties include cell culture, retina histology (e.g. sectioning
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: Immediately available Group or Departmental Website: https://med.stanford.edu/ophthalmology.html (link is external) How to Submit Application Materials: Please send application materials to Dr. Ahmad Al
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of Molecular Medicine and Therapeutics at The Ohio State University College of Medicine. Lab website: http://u.osu.edu/mathur Our goal is to use the lens of metabolism to better understand and predict cancer
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, manuscript drafts, and research proposals. Required Skills and Abilities 1. Ability to independently design, execute, and troubleshoot molecular biology experiments (e.g., cloning, PCR, RNA analysis, genome