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departmental laboratories 5) Working with departmental instructional staff and providers to maintain several key common pieces of molecular equipment, such as PCR machines, imagers, hoods, autoclaves, nanodrops
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to date, maintain equipment, routine molecular biology (e.g. PCR/qRT-PCR, Western blotting) and maintenance of databases. Additional duties include cell culture, retina histology (e.g. sectioning
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sequencing workflows in both fundamental research and clinical studies. Visit: https://chowdhurylab.dana-farber.org/ https://www.dana-farber.org/cancer-care/treatment/brca-related-genes Located in Boston and
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characterization of the iPSCs and neuronal models via: Immunostaining, cryosectioning, and microscopy DNA/RNA extraction (q)RT-PCR Western blotting Generation of cell lines using CRISPR-Cas9 gene editing methods
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, harvests), multi-parameter flow and mass cytometry (CyTOF), fluorescence-activated cell sorting (FACS), multiplexed fluorescence microscopy and single-cell RNA sequencing; prepare RNA and DNA; perform PCR
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target identification, dsRNA construct design, and delivery optimization Carry out molecular and genomic work, including sequence analysis, primer design, PCR, dsRNA synthesis, RNA extraction, cDNA
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: Immediately available Group or Departmental Website: https://med.stanford.edu/ophthalmology.html (link is external) How to Submit Application Materials: Please send application materials to Dr. Ahmad Al
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isolation, PCR, gel electrophoresis, Westerns) Ability to work well within the lab group and with collaborators. Preferred Qualifications Mouse Embryonic Stem Cell (mESC) culture experience. Experience
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will include animal studies, sterile cell culture techniques, PCR, restriction digest, plasmid purification, gel electrophoresis, and maintaining a detailed lab notebook containing research data. Assists
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of human cell lines, human Peripheral Blood Mononuclear Cells (PMBCs), electroporation, transfection, and transduction. Basic molecular biology techniques that include PCR, DNA cloning, plasmid design and