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electrophoresis (in agarose gel, SDS-PAGE), PCR, Western Blot, proteomics, metabolomics bioinformatics, statistical analysis, database management, use of specialized software such as R, Proteome Discoverer, Mascot
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precisely. Tasks and responsibililties Collecting and processing umbilical cord blood from the operating room and delivery rooms. Sterile isolation and culturing of primary cells and cell lines. PCR analysis
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preparation, cell culture, bacterial culture, ELISA, immunoblotting, and PCR-based methods. Maintain and manage mouse colonies, including animal handling, monitoring health and breeding, genotyping
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basic dry and wet chemicals and other common laboratory reagents. Perform conventional PCR and quantitative RT-PCR. Conduct immunoblotting and related assays. Maintain cell lines and primary cells in
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tissue culture experiments and basic molecular biology techniques (PCR, plasmid construction) as well as basic microscopic skills. The candidate should be able to work with scientific literature and
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techniques, including DNA and RNA extraction, PCR, real time PCR, droplet digital PCR, and other techniques Process human biological samples, including isolation of cells from blood and tissues Culture cells
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microbiology techniques, molecular biology including PCR and DNA sequencing, and clinical research skills including execution of prospective cohort studies and clinical trials. Grant and manuscript preparation
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utmost importance. Additional duties include database and sample tracking management, data analysis and interpretation, and quantitative PCR. The applicant will have the option to also assist with
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: Genotyping mice using PCR. Maintaining records of the transgenic mouse collection. Managing the breeding of over 30 transgenic lines. Coordinating communication between the research team and animal facility
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: 87384BR This is a training position. The Trainee will be enrolled in a state-approved training program for the Clinical Histocompatibility Scientist (CHS). (https://www.cdph.ca.gov/Programs/OSPHLD/LFS/Pages