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basic dry and wet chemicals and other common laboratory reagents. Perform conventional PCR and quantitative RT-PCR. Conduct immunoblotting and related assays. Maintain cell lines and primary cells in
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University of California, San Francisco | San Francisco, California | United States | about 1 month ago
experiments under supervision initially and then more independently and carry out general laboratory maintenance tasks. Experiments will include basic molecular biology experiments, including PCR, cloning
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: 87384BR This is a training position. The Trainee will be enrolled in a state-approved training program for the Clinical Histocompatibility Scientist (CHS). (https://www.cdph.ca.gov/Programs/OSPHLD/LFS/Pages
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required. Experience with cell culture is needed. Experience with viral vectors including retroviral vectors, nucleic acid extraction and PCR is beneficial. A strong interest in and commitment to advancing
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of standard immunological techniques and molecular biology assays including: ELISA, flow cytometry, Western Blot, PCR, RT-PCR, agarose gel electrophoresis, DNA isolation, RNA isolation, protein purification
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cytometry and routinely applies core molecular biology techniques, including Western blotting and quantitative PCR (qPCR). Prepares written reports of the research experiments. Prepares grant proposals based
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of lentivirus/adenovirus/adeno-associated virus, cell transduction, cell line generation, live cell imaging, histology, microscopy, biochemical assays in cells, isolated mitochondria and tissue homogenates, PCR
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routine laboratory experiments such as performance and analysis of real-time PCR, western blotting, biochemical assays, immunohistochemistry, immunofluorescence, EIAs, gene transfection/transduction studies
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-up procedures). · Performance of basic molecular biology techniques (e.g. end-point and real-time PCR, gel electrophoresis, mammalian tissue culture, setting up reagents, total RNA, genomic DNA
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culture and molecular bench work including, DNA and RNA isolations, PCR reactions, and ELISA assays. The successful candidate also needs to be interested in learning the imaging analyzing skill and analysis