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, including primary cell isolation and culture Proficiency with molecular biology techniques, including qRT-PCR, Western blot, immunofluorescence Microscopy skills (fluorescence, confocal, live-cell imaging
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qualifications Demonstrated competence with core molecular biology techniques (including vector design, genome-targeting strategies, and PCR-based workflows). Experience with viral delivery, transduction
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Additional experience with behavioral assays preferred. Proficiency with various molecular biology techniques such as PCR and RT-PCR, DNA and RNA extraction, Sanger sequencing analysis, western blotting
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genetic characterization. This individual should also know how to implement and troubleshoot PCR protocols including Single Genome Amplification, next generation sequencing library preparation, sequencing
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agreement. The salary range for this position is $30.89 - $49.68 (Hourly Rate). To learn more about the benefits of working at UCSF, including total compensation, please visit: https
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is $28.04 - $45.09 (Hourly Rate). To learn more about the benefits of working at UCSF, including total compensation, please visit: https://ucnet.universityofcalifornia.edu/compensation-and-benefits
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: One year of relevant research experience. Molecular Biology techniques (i.e. quantitative real-time PCR, RNA and DNA isolation, transfection, Lentivirus mediated gene silencing, transformation, cloning
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, including collecting, labeling, and processing biological samples for testing. Setting up experiments, running protocols (e.g., cell culturing, DNA extraction, PCR, ELISA, and staining slides and microscopy
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, generation, troubleshooting, and validation of novel genotyping strategies for newly engineered mouse alleles and genetically modified cell lines. • Evaluate and quantify gene expression using quantitative PCR
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will include, but are not limited to, studies of virus-host interactions using HIV-1 as a system model. Projects include analyzing HIV-1 interactions with neutralizing antibodies using viral assays, PCR