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, PCR-based assays, and metagenomic sequencing. Design and optimize PCR primers and probes; conduct PCR, qPCR, and digital PCR assays with troubleshooting as needed. Prepare next-generation sequencing
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | about 4 hours ago
preparation for and/or assistance with PCR genotyping, western blotting, DNA cloning 2) Preparation of solutions and standard media 3) Assist PI/other lab members with anesthetizing mice and dissection
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microscopes. Isolation of protein, RNA, and DNA from cultured cells and isolated murine ocular tissues with standard methods for western blot, PCR, and ELISA assays; Statistical and image analyses with various
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dependencies, and improving radiation therapy efficacy. What You’ll Do: - Maintain detailed and organized lab notebooks documenting experiments and mouse colony work. - Perform PCR, RT-PCR, Western blots, and
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Tranplantation and Regenerative Medicine Postdoc Appointment Term: 1-year term (Renewal is based on performance -- Max of a 5 years) Appointment Start Date: As Soon As Possible Group or Departmental Website: https
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should be proficient in common bio-medical laboratory procedures such as tissue homogenization, Western Blotting, RT-PCR, ELISA, common biochemical assays for determining DNA, Protein, lipids, Individual
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. Organisational and project-management skills, including a strong record of meeting tight deadlines. Hands-on experience in PCR and qPCR assay design, optimisation, setup, and data analysis, including
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basic and moderately complex experiments using Molecular biology techniques (PCR, gel electrophoresis, DNA cloning) CRISPR techniques to disrupt targeted genes Microinjection, manipulation, and
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of the position will help with the breeding, handling, and euthanizing of mice; amplifying DNA molecules by PCR, using various cloning procedures to generate DNA constructs, performing cell culture, and maintaining