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genome encodes gene expression levels. You will undertake large scale data generation from primary human samples using a method recently pioneered by the host laboratory (Hua et al., Nature 2021 https
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perform experiments using an array of state-of-the-art techniques from systems neuroscience, genetics, and physiology. More information about this lab can be found on his website https://knightlab.ucsf.edu
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given to candidates with: Experience in the areas indicated above; Scientific publications in areas related to the APTA4shiga project; Experience in molecular biology, including conventional PCR and real
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implementation of molecular markers in plant material. Documented experience in laboratory work, including planning and conducting research based on PCR and qPCR techniques, as well as DNA sequencing using DArTseq
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animals; store and analyze the tissue (e.g., ESLISA kits, PCR, immunohistochemistry, estrous classification). Prepare buffers, reagents, and other solutions and test for pH level. Keep meticulously detailed
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equipment used by multiple researchers (e.g., DNA and RNA extraction rooms, PCR machines, instruments to measure concentration and integrity of nucleic acids, and knowledge of best practices for down-stream
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for microscopy or RNA-sequencing. · Performing histological sectioning and immunofluorescence histochemistry on rat brain tissue. · Performing immunoprecipitation of tagged proteins, q-PCR, and
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advance notice. Preferred Qualifications: Experience working with animals in a research setting. Experience with molecular biology techniques (PCR, qPCR, western blots). Position Sponsorship Eligibility
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prefered. Experience in animal handling, mouse blood and organ collection, molecular cloning, cell culture, immunoblot analysis, immunohistochemistry staining, PCR, and RNA extraction. Mouse tail vein inject
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throughput genomic sequencing), quantitative PCR, RNAseq, assistance with animal work, Gene gun immunization, protein analysis (Expression, purification, Westerns, ELISAs, IPs, Mass Spectrometry analysis) and