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- Institute of Physical Chemistry, Polish Academy of Sciences
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- Institute of Biochemistry and Biophysics Polish Academy of Sciences
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of methods mentioned below: cell culture, generation of reporter cell lines, working with primary cells, lentiviral transductions cell sorting preparing sequencing libraries for Illumina (RNAseq, ChIPseq
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has resulted in several failed clinical interventions for human diseases. Similarly, large-scale sequencing datasets, while informative, do not fully elucidate human neutrophil functionality
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project aims to address this challenge by developing sequence-programmable polymeric materials designed for long-term data storage. By harnessing the potential of synthetic polymers with precisely
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to perform CUT&RUN analysis of genes regulated by NRF2 isoforms in selected cell models. That also requires the preparation of DNA library for the NGS sequencing. Another aspect to study during this time is to
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sequencing. Has the ability to communicate (in English) complex information clearly, orally and in writing. Has good presentation skills (ideally having presented during at least one international conference
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organoids, advanced genome editing techniques (inhibition and activation of expression) including screening studies, RNA sequencing at the single-cell level, Perturb-seq, functional tests of beta cells, and
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sequencing, proteomics and lipidomics, functional assays, and microscopic imaging. Mastering all of these techniques is not necessary, as the project will be carried out in collaboration; however, there is an
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studying Pol I and Pol III structure and transcription regulation by teaming up to explore Pol biogenesis. We will identify the sequence and mechanisms of subunit assembly. We postulate that early Pol I and
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controlled monomer sequences that can self-organize into complex supramolecular structures. The research aims to understand the mechanisms of single-chain folding and hierarchical self-organization, enabling
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differentiation of human stem cells into pancreatic beta cells • Creation of pancreatic organoids • Advanced genome editing (CRISPR, CRISPRi/a) • High-throughput screening • Single-cell RNA sequencing