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- Institute of Physical Chemistry, Polish Academy of Sciences
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of methods mentioned below: cell culture, generation of reporter cell lines, working with primary cells, lentiviral transductions cell sorting preparing sequencing libraries for Illumina (RNAseq, ChIPseq
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has resulted in several failed clinical interventions for human diseases. Similarly, large-scale sequencing datasets, while informative, do not fully elucidate human neutrophil functionality
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of peptide linkers for the modification of multicomposite polymeric materials. The candidate will be responsible for obtaining peptide(s) or peptide mimetics with a specific sequence, modifying them with
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organoids, advanced genome editing techniques (inhibition and activation of expression) including screening studies, RNA sequencing at the single-cell level, Perturb-seq, functional tests of beta cells, and
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studying Pol I and Pol III structure and transcription regulation by teaming up to explore Pol biogenesis. We will identify the sequence and mechanisms of subunit assembly. We postulate that early Pol I and
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sequencing, proteomics and lipidomics, functional assays, and microscopic imaging. Mastering all of these techniques is not necessary, as the project will be carried out in collaboration; however, there is an
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controlled monomer sequences that can self-organize into complex supramolecular structures. The research aims to understand the mechanisms of single-chain folding and hierarchical self-organization, enabling
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differentiation of human stem cells into pancreatic beta cells • Creation of pancreatic organoids • Advanced genome editing (CRISPR, CRISPRi/a) • High-throughput screening • Single-cell RNA sequencing
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Institute of Animal Reproduction and Food Research of Polish Academy of Sciences | Poland | 3 months ago
, ChIPmentation). Analysis and integration of high-throughput sequencing data. Position 2: Epigenetic Memory in Immune Cell Responses to Vitamin D Project: Research on epigenetic memory mechanisms based
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genomics, whole-genome sequencing Project topics: Climate change is proceeding at an unprecedented rate, which leads to a considerable loss of biodiversity that may disturb the functioning of ecosystems