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metagenomics protocols using third-generation sequencing technologies, from the collection of biological samples to the biological validation of a result. The thesis project (IHAP and MIAT labs) will focus
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of sequencing libraries for illumina and nanopore sequencing. The successful candidate will convert the RNA into molecules compatible with high-throughput sequencing by adding barcodes and sequencing adapters and
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reporter protein. The droplets exhibiting the phenotype of interest will then be sorted, their DNA content recovered and analyzed by high-throughput sequencing to characterize the result of each screening
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based on a circularly permuted green fluorescent protein (cpGFP) inserted into the intracellular loop 3 sequence of the receptor endogen (RXFP3). This prototype will be optimised by multiple cycles
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will be to generate the mitogenomic data and, then, to integrate and analyze all datasets. -- Generation of mitogenomic data (high-throughput sequencing and bioinformatic analyses) -- Phylogenetic and
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sequencing, and mass spectrometry. With multidisciplinary research teams and a dynamic setting, the IBMC offers a stimulating environment conducive to scientific innovation. The IBMC is also affiliated with
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, the PhD student will have at his/her disposition a set more than 150 high quality genomes (assembled from long-read sequencing and polished with short-reads), more than 100 of P. destructans and more
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analyses (bioinformatic and statistical) of environment and microbiome data, including amplicon sequencing libraries, metagenomes, and metatranscriptomes. • Publish (as lead and co-author) in leading
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to macroevolutionary questions related to a better understanding of the tree of life, will involve the development and application of complex sequence evolution models (mixture models) and molecular dating, within both
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for degradation, the PTI response is nucleotide sequence-independent and triggered also by other dsRNA molecules, for example by exogenous treatment of plants with poly(I:C) (Niehl et al., 2016, New Phytol 211