Research Specialist - CryoEM

Summary:

The HHMI CryoEM Shared Resource at Janelia Research Campus is a world-class core facility with a large and diverse user base. It serves the entire HHMI community nationwide for cutting-edge research in structural and cellular biology. The facility currently hosts three highly configured Titan Krios microscopes with a 4th Krios microscope being installed, an Aquilos2 cryo focused ion beam scanning electron microscope (FIBSEM), a Hydra plasmon cryo FIBSEM, multiple screening electron microscopes, cryo light microscopes, and cryo grids preparation equipment.

The main function of the facility is 1) high-throughput, high-quality data collection on user-provided ready-to-image cryo grids utilizing either single particle or cryo-electron tomography; 2) use the cryo FIBSEM system to thin down thick samples for cryoEM imaging; 3) collaborate on research projects with HHMI labs when needed; 4) implement and test new methods/protocols to better help users.

About the role:

In this role, you will play a crucial part in enabling breakthrough discoveries by supporting high-throughput data collection, assisting in sample screening and data handling, and ensuring the smooth operation and maintenance of the CryoEM facility’s workflows and equipment. Working under the guidance of senior staff members, you will participate in the implementation of new workflows and protocols, collaborate with users to plan, execute, and share experimental results, and help maintain equipment and train users on applicable facility tools and software. Your technical contributions will be essential to the success of collaborative scientific projects and advances in structural biology.

What we provide:

• A competitive compensation package, with comprehensive health and welfare benefits.

• Interdependent, team-wide support in daily operations; hands-on training and mentorship.

• Access to world-class researchers, resources, and impactful science as part of your career development.

• The opportunity to contribute your expertise to a shared resource that enables high-quality research.

• Professional development opportunities through internal and external conferences and workshops.

• A healthy work-life balance, with on-site childcare, free gyms, on-campus housing, vibrant social and dining spaces, and shuttle-bus service to Janelia from the Washington, DC metro area!

What you’ll do:

• Provide high-quality, high-throughput data collection services to users, following established protocols.

• Help screen users’ samples and provide feedback.

• Properly handle and manage various samples, sample storage systems, cryogen dewars, and dry shippers.

• Assist with managing, storing and transferring users’ data.

• Maintain and operate various equipment from sample preparation systems to Krios microscopes.

• Train new users on basic equipment and workflows.

• Log and report hardware and software issues, solve problems when applicable or escalate concerns to senior team members as appropriate.

• Participate in the implementation of new protocols and methods for sample preparation, screening, instrument operation, data collection and data curation.

• Expand own skill set and stay current on relevant techniques to support facility workflows.

• Communicate effectively with users and colleagues to coordinate data collection and report routine results. Carry out other assigned tasks not mentioned above

What you bring:

• Master’s degree in a relevant scientific field and 4+ years of related research experience, or an equivalent combination of education and experience.

• Experience with cryoEM or electron microscopy are preferred.

• Background in biochemistry, protein purification, or cell culture is a plus.

• Eagerness to learn and apply new techniques, protocols, and technologies.

• Ability to work in a highly interactive, and rapidly changing environment.

• Excellent communication and interpersonal skills and ability to work closely and collaboratively with colleagues and users.

• Enthusiastic to take on new challenges.

• Good knowledge and practical skills with computers, linux operating system, bash scripting and simple programming in Python is a plus.

Physical Requirements

Remaining in a normal seated or standing position for extended periods of time; reaching and grasping by extending hand(s) or arm(s); dexterity to manipulate objects with fingers, for example using a keyboard; communication skills using the spoken word; ability to see and hear within normal parameters; ability to move about workspace. The position requires mobility, including the ability to move materials weighing up to several pounds (such as a laptop computer or tablet). Work may involve possible exposure to malodorous vapors, low dose radiation, contamination by toxic chemicals and acids and presence of carcinogenic substances or other hazardous materials.

Persons with disabilities may be able to perform the essential duties of this position with reasonable accommodation. Requests for reasonable accommodation will be evaluated on an individual basis.

Please Note:

This job description sets forth the job’s principal duties, responsibilities, and requirements; it should not be construed as an exhaustive statement, however.  Unless they begin with the word “may,” the Essential Duties and Responsibilities described above are “essential functions” of the job, as defined by the Americans with Disabilities Act.

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