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/practicals. Experience with C. elegans (husbandry/genetics/transgenics) Experience in molecular cloning and genome editing Experience in microscopy, including confocal microscopy, live imaging and image
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cultures. Experience in molecular biology methodologies, following and adapting protocols and setting up cellular assays including image-based is essential for this role, and knowledge of neuroinflammation
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research, fundamental cell biology and cell signalling with excellent shared facilities including the Centre for Proteome Research, the Computational Biology Facility, the Liverpool Centre for Cell Imaging
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well as bioinformatics and bacterial imaging. Main Duties and Responsibilities Perform the following activities in conjunction with and under the guidance of the Principal Investigators (PI): 1. Plan and conduct assigned
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, intracranial injections of viral vector (to allow optogenetic/chemogenetic manipulation), electrophysiological experiments (single cell neurophysiology) and histology/imaging. You will be based in Dr Sollini’s
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metallodrugs Mammalian cell culture including experience of 3D cell culture techniques. Confocal microscopy including quantitative image analysis Flow cytometry General molecular biology techniques such as
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group aims to determine regulatory pathways affected by disease by implementing the use of spatial proteomics combined with transcriptomics and live imaging. The total proteome of a neuron includes a vast
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, stable isotope tracers, medical imaging). Experience with molecular biology techniques, stable isotope tracers methodologies, GCMS desirable. -Familiarity with bioinformatics and computational biology
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responsibility for implementing a deep learning work-package as part of a Cancer Research UK-funded programme, developing an image-recognition model to identify morphological features corresponding to clonal
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to target specific transcription factors (iii) use of high content imaging and AI to phenotype these cultures (iii) use of bulk and single-cell RNAseq to characterise the transcriptional profile of each cell