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lab uses circuit dissecting ex vivo and in vivo electrophysiology, fiber photometry, viral based CRISPR/Cas9 gene-editing, and methods to manipulate neural activity in animal models of stress and
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for collection, verification, statistical analysis, and management of data. Experiments would include cell culture, molecular biology (including gene editing), antibody production and engineering, flow cytometry
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-leading research in several fields. Notably, the groundbreaking discovery of the CRISPR-Cas9 gene-editing tool, which was awarded the Nobel Prize in Chemistry, was made here. At Umeå University, everything
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following disciplines: bacterial genetics, molecular cloning, gene editing approaches, host–pathogen interaction models, immunological assays (including cytokine analysis, flow cytometry, ELISA, Western
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gene editing, single cell RNA sequencing, bioinformatics, genetic engineering in mice, intravital microscopy and other imaging modalities, and a number of cell and molecular techniques. For more
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in Chlamydomonas using CRISPR-Cas9 gene editing approaches (ii) Spectroscopic characterisation of growth and photosynthesis in the mutant cells (iii) Biochemical purification of tagged complexes. (iv
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PCR, ELISA; microscopy; flow cytometry; immunostaining; and experience with animals (i.e., mouse handling, organ collection). Experience in genetic modification techniques, including gene editing (e.g
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applicant will drive a multi-target gene-editing screen in Populus to pierce redundancy and reveal secondary cell wall phenotypes, followed by mechanistic characterisation of candidate genes through in situ
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University of Massachusetts Medical School | Worcester, Massachusetts | United States | 3 months ago
of these candidates to determine how they regulate pericyte specification. For all projects, we routinely develop and apply cutting-edge approaches, including conditional genetics, CRISPR-based gene editing, single
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-leading research in several fields. Notably, the groundbreaking discovery of the CRISPR-Cas9 gene-editing tool, which was awarded the Nobel Prize in Chemistry, was made here. At Umeå University, everything