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vesicles, cancer vaccines, brain cancer, motor neuron disease, protein corona, hydrogels, and gene therapy/editing. Hands-on experience in advanced cell culture techniques and in vivo models. Proficiency in
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skills are particularly welcome. For information about the lab and the PI, please visit: https://www.cellbio.duke.edu/purushothama-rao-tata/ . This will be a minimum one-year appointment, contingent upon
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determining whether cargo, e.g., CRISPR/Cas9 gene editing machinery, reaches the cytosol or is degraded. Overcoming this barrier is crucial to unlock the full potential of EVs as Advanced Therapy Medicinal
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to possess a strong academic background or research experience in one or more relevant disciplines, including cellular therapy, extracellular vesicles, gene editing, gene therapy. ABOUT COLLEGE OF HEALTH
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assist with the preparation of annual evaluations. Process Letter of Appointment and student terminations and edit jobs. Maintain college contact list and distribute accordingly Maintain tracking system
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completion of assigned tasks. The Research Coordinator will generate gene-edited cavefish and assess their behavior, while collaborating with other researchers to analyze resulting phenotypes. Opportunities
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microRNA signaling in fetal growth restriction, and (2) pioneering in utero gene editing approaches to correct genetic disease before birth. Current projects include the development of CRISPR-Cas9–based base
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). Bioinformatics exposure (proteomics, RNA-seq, phylogenetics). Biochemical purification, including FPLC and SEC-MALS. Mammalian and insect cell culture and transfection. CRISPR/Cas9 gene editing. Physical
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RNA isolation, siRNA-mediated gene knockdown in cell lines and primary cells, molecular cloning, and imaging. Experience with CRISPR/Cas9 gene editing and CRISPR screening is an advantage, as is prior
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, nanoparticle formulation, peptide therapeutics, T cells therapy, extracellular vesicles, cancer vaccines, brain cancer, motor neuron disease, protein corona, hydrogels, and gene therapy/editing. Hands