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and training the center users on various microscopy systems including wide-field, TIRF, confocal, multiphoton, two-photon in vivo imaging, FLIM, and FRET microscopy. The incumbent expected to have some
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Ministero dell’Università e della Ricerca (MUR)-fondi dell’Unione Europea – programma NextGenerationEU. | Italy | 14 days ago
Resonance Energy Transfer (FRET). The main objective is to investigate how a suitably engineered local electromagnetic environment can modulate, in a controlled manner, the radiative and non-radiative
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and cellular drug screening assays to evaluate small molecule inhibitors. Assays include imaging, TR-FRET, inhibitor/target interaction studies, cell culture, automated nanoLt dispensing and more
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the Molecular Crowding of Ligand-Induced Riboswitch Folding: Kinetic/Thermodynamic Insights from Single Molecule FRET Spectroscopy”, J. Phys. Chem. 126, 6419–6427 (2022) https://doi-org.colorado.idm.oclc.org
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computational analyses of large -omics datasets. Some of the techniques we use include standard cell and molecular biology techniques, flow cytometry, microscopy (standard imaging and FRET, FLIM, FRAP), high
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) effective communication 3) critical evaluation of data and research articles, and 4) basic-to-advanced laboratory skills encompassing cell to animal physiology (cell isolation and culture, FRET and calcium
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assays by biophysical methods (SPR, MST, ITC, BLI) or Fluorescence-based (i.e. FP, TR-FRET) methods, and optimizing workflows for ligand discovery. Depending on your skills, you might also work
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-molecule FRET. The interdisciplinary approach of this project provides scope for students to shape the project, which is enhanced by specialist networks in the University of Sheffield, including the Nucleic
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purification, biochemical and cellular assays Reconstitution of multi-protein and protein:RNA complexes Assay development Cryo-electron Microscopy SAXS FRET; FRET-FLIM Super-resolution microscopy The control
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, experimental design and data analysis methods. In this project, you will use the following techniques: Cutting edge microscopy (confocal and FRET) to image biosensors to detect hormone and sugar concentrations