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-on experience with advanced techniques like patch-clamp fluorometry (PCF), Förster resonance energy transfer (FRET), and the use of fluorescent noncanonical amino acids (ncAAs). Lipid-mediated regulation of ion
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biochemistry to join a newly funded GPCR collaborative project to investigate the structural dynamics of GPCRs and their signaling partners using biophysical methods, including single-molecule FRET (smFRET
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-molecule transport and measure the NPC nanoscale structure using TEM, AFM, FRET, DIB, MINFLUX, etc. We study purified yeast NPCs as well as design the amino-acid sequence of the IDPs and TRs de novo from
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using TEM, AFM, FRET, DIB, MINFLUX, etc. We study purified yeast NPCs as well as design the amino-acid sequence of the IDPs and TRs de novo from the bottom up and study these in biomimetic solid-state
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | 1 day ago
fluorescence polarization, time-resolved FRET (TR-FRET), FRET, and BRET. Proficiency in biophysical assay platforms, including isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR), is also
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engineering to measure the structural and dynamic properties of individual biomolecules using spectroscopic techniques such as single-molecule Forster Resonance Energy Transfer (FRET) and cryogenic electron
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workflows of bioimaging analysis to quantify advanced imaging data generated by super-resolution STED microscopy, FLIM, FRET and FCS. Candidates must hold a PhD in cell biology, biophysics or biochemistry
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data generated by super-resolution STED microscopy, FLIM, FRET and FCS. Candidates must hold a PhD in cell biology, biophysics or biochemistry along with experience in advance quantitative microscopy
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Fluorescence, FRET, & single‑molecule microscopy Mechanobiology, force-spectroscopy Bioconjugation chemistry, nucleic‑acid chemistry Quantitative image analysis, MATLAB/Python scripting, or machine learning
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, particularly with protein ligation and conjugation techniques + Experience in mammalian cell culture, protein/peptide mass spectrometry, and fluorescence-based techniques (e.g., FRET, nano-BRET) is highly