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ESPCI Ecole supérieure de physique et de chimie industrielles de la ville de Paris (PSL) | Paris 15, le de France | France | 29 days ago
de microscopie capable de détecter simultanément des molécules fluorescentes individuelles ainsi que leur durée de vie de fluorescence, permettant ainsi d'obtenir des images super-résolues de la durée
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fluorescent proteins, SNAP-tag labeling, and related cell-based imaging approaches. • Prepare samples for and perform experiments using super-resolution microscopy and single-molecule fluorescence imaging
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paradigm shift: imaging through time rather than space. We have recently demonstrated that the position of fluorescent molecules can be reconstructed from a temporal encoding of their emission, using a
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omics platforms (e.g., fluorescence microscopy, mass spectrometry imaging (MSI), Raman systems) and will develop computational and statistical data analysis methods to improve data quality, information
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endoscopic head [2]. This setup allows for multiphoton imaging (2-photon fluorescence, second harmonic generation - SHG) over a 400 µm field at a rate of 10 images per second with micron-level lateral and
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-resolution microscopy and/or single-molecule fluorescence imaging applied to biological problems in cells. • Proven experience in quantitative analysis of membrane protein organization, oligomeric states, or
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imaging methods for temperature sensing. Despite recent progress, temperature-dependent fluorescence sensing is still underexplored. Our goal is to investigate the photophysical mechanisms behind
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on destructive assays or fluorescent labeling, which limit continuous monitoring of cell cultures. Label-free approaches represent a promising alternative. Hyperspectral imaging (HSI) enables the extraction
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and tumor tissue) to determine their immune cell infiltration profiles 3D imaging analyses using fluorescence microscopy techniques (e.g., confocal laser scanning microscopy, light-sheet fluorescence
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HBIGS Heidelberg Biosciences International Graduate School | Heidelberg, Baden W rttemberg | Germany | about 23 hours ago
and temporal dynamics of phyB PBs relative to nucleosomes using live imaging (phyB and H2B fluorescent lines) and MINFLux super-resolution microscopy at the 10-50 nm scale (using SNAP-tagged phyB and