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to develop a new generation of fluorescence microscopy methods aimed at overcoming current limitations in acquisition time for single-molecule imaging. The project explores original strategies for temporal
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, with a strong interest or expertise in photophysics -knowledge of fluorescence microscopy -experience in single-molecule imaging, including data acquisition and analysis -familiarity with biological
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neurons. Using a combination of high resolution live cell fluorescent imaging, electrochemistry and patch clamp electrophysiology, we recently discovered that activation of GLP-1Rs promotes the formation
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secretion from chromaffin cells. Using a combination of patch clamp electrophysiology and live cell fluorescent imaging the interactions between heterotrimeric G protein subunits, their effectors and the
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the Light Microscopy Facility (https://www.sheffield.ac.uk/lmf). Wild-type and mutant lines will be crossed to transgenic lines of interest for live fluorescence imaging. There will also be the opportunity
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viral injections, basic programming skills, electrophysiology, or histology, and RNA-sequencing. Job Duties Collect large scale neural recordings using multiphoton fluorescence imaging as
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cooperation partners in their research projects with state-of-the-art light and electron microscopes (https://www.leibniz-fmp.de/cellular-imaging ). We work with proteins, cell cultures, and model organisms
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following: Confocal or live-cell fluorescence imaging Molecular cloning and transgenesis Single-cell RNA sequencing or transcriptomic analyses Experience in marine invertebrate model systems. Demonstrated
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-imaging. As part of this project, the candidate will be working on: 1: Labelling and live-imaging distinct types of cell death within the optically translucent lifestages of Drosophila (embryo and pupa
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tools for interfacing with the brain, including genetically encoded voltage indicators (GEVIs), optogenetic silencers, and fluorescent proteins. We welcome candidates with diverse expertise, which may