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, for advancing our fluorescence-based DyeCycling/FRET technology to study biomolecular dynamics. Biomolecular dynamics, such as conformational changes, are the understudied link between biomolecular structure and
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-bleaching of the fluorescent dyes involved, which ends the experiment prematurely, rendering many biological questions inaccessible. To bypass this limitation, our group has developed DyeCycling/FRET, where
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collaboration to combine their expertise. By integrating these complementary approaches, we aim to provide the first comprehensive picture of Hsp90's structure–dynamics–function relationship, with broad
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