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relevant in gene-regulation. Tasks: Cloning and production of recombinant proteins from E. coli and of RNA by means of in vitro transcription Qualitative/quantitative analyses of protein-RNA/DNA complexes
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to prevent and/or mitigate the impact of E. coli and Klebsiella spp. infections, including those associated with antimicrobial resistance (AMR). As part of this post there is an opportunity to analyse large
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activates its specific target in Pseudomonas aeruginosa, we will design, develop and test novel anti-biofilm compounds with broader activity against the WHO priority pathogens Escherichia coli, Klebsiella
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. This position will focus on: (1) sustaining the long-term evolution experiment with E. coli; (2) employing and refining existing methods to measure phenotypic and genomic changes in this and other similar
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recombinant proteins from E. coli , yeast, cell cultures, and/or plants Perform protein–protein interaction assays such as Co-immunoprecipitation (Co-IP), yeast two-hybrid (Y2H), Biolayer Interferometry (BLI
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. You will use this approach to determine how existing inhibitors affect E. coli DNA polymerase I, then apply it to Staphylococcus aureus (MRSA) polymerase polC to explore how experimental antibiotics
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for the production of well characterised tau samples for use in mechanistic studies across the UK DRI. This will include: Expression, purification and quality control of recombinant tau from E. coli and mammalian
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sources Structure determination of biomolecules by NMR, Mass Spectroscopy, Dynamic Light Scattering, etc. Engineering and expressing recombinant proteins using various hosts (E. coli, yeast, mammalian cells
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and the Bio-Nano Sensing group on the expression, modification and testing of new protein-based sensing systems. The successful candidate will lead on the design, recombinant expression (E. coli ) and
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limited to salmonella, Listeria, E. coli, Campylobacter, hot acids, bases, ethers and other chemicals. Special Requirements – Please contact your recruiter with questions regarding which activities apply by