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The Absmeier group (https://www.bcp.fu-berlin.de/en/chemie/biochemie/research-groups/absmeier-group/index.html ) is interested in the regulation of mRNA translation and decay. We recombinantly
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like CRISPR systems Experience in strain engineering workflows e.g., Pseudomonas putida, E. coli or Cupriavidus necator. Experience in handling automation instruments for different molecular biology
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sources Structure determination of biomolecules by NMR, Mass Spectroscopy, Dynamic Light Scattering, etc. Engineering and expressing recombinant proteins using various hosts (E. coli, yeast, mammalian cells
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yeast), allowing them to monitor the fate of this lesion in vivo. The proposed postdoctoral research project will explore the genetic and molecular mechanisms of DNA damage tolerance pathways in E. coli
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biosynthesis in Kitasatospora purpeofusca. The successful candidate will be responsible for characterizing the biosynthesis gene group. - Cultivate bacterial strains (Streptomyces, Kitasatospora/Escherichia coli
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for engineering synthetic gene circuits to program temporal tasks in E. coli cells. This position is funded by an ERC Starting Grant (project: TICK-TOCK Do and Die). Past achievements of Dr. Santos-Moreno include
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researcher for engineering synthetic gene circuits to program temporal tasks in E. coli cells. This position is funded by an ERC Starting Grant (project: TICK-TOCK Do and Die). Past achievements of Dr. Santos
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, Biochemistry, Metabolic engineering, Applied Microbiology, Microbial Biotechnology or other relevant to this application. Experience with molecular biology, microbiology and advanced E. coli and/or yeast
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working with diverse host organisms (e.g., E. coli, yeast, mammalian cells) is a significant plus. Deep expertise in advanced genome engineering tools (e.g., CRISPR/Cas systems, base editing, prime editing
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enteric pathogens (mainly E. coli and Salmonella Typhimurium) to uncover novel resistance mechanisms and identify new antimicrobial targets. We employ molecular biology, bacterial genetics, chemical