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follow-up (e.g., CRISPR/Cas9 strategy, genotyping workflows, mutant/variant assessment) Comparative frameworks (orthology-aware thinking, cross-species mapping, or phylogenetic reasoning) General
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. Strong experience with cellular engineering, including CRISPR gene editing and synthetic biology approaches. Profound experiences in antibody and fusion protein engineering, click chemistry and display
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characterization of the iPSCs and neuronal models via: Immunostaining, cryosectioning, and microscopy DNA/RNA extraction (q)RT-PCR Western blotting Generation of cell lines using CRISPR-Cas9 gene editing methods
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clones and complex constructs.•Use of lentiviral systems for gene delivery and functional studies.•Application of CRISPR/Cas technologies for genome editing and functional validation.•Recombinant protein
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QUALIFICATIONS: Background in stem cell biology, cancer biology, molecular biology, and/or immunology. Expertise with molecular biology techniques such as DNA/RNA isolation, PCR, qPCR, CRISPR/Cas9, and/or cloning
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cardiovascular and metabolic research. Learn more about our school (https://www.kcl.ac.uk/scms ) Our research spans the fundamental molecular, cellular, and physiological mechanisms underlying normal and
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, CRISPR-Cas System, Data Analysis, Data Interpretations, Flow Cytometry, Genome Editing, Hematology, Immunology, Injection Techniques, Mouse Handling, Stem Cell Biology, Verbal Communications, Written
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will have expertise in molecular and cellular biology and will notably perform labelling and imaging by STED of primary culture, modification of the cells by CRISPR-Cas9 to introduce mutations, and
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culture and molecular biology techniques such as CRISPR gene editing. Certifications/Licenses Required Knowledge, Skills, and Abilities Must be able to follow detailed laboratory instructions. Preferred
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and dopamine recording with fiber photometry Chemogenetic DREADDs Optogenetics CRISPR/Cas9 Other molecular genetic and gene transfer approaches in Syrian hamsters. Description of Lab Overall