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as molecular tools, and applying these novel tools in disease models. Recently, we are actively working on RNA-guided systems, such as the well-known CRISPR-Cas, which is widely used for genome editing
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the molecular regulation of dendritic cell subsets, function, and differentiation using novel approaches, such as CRISPR Cas9, single-cell and tissue analyses. The work will contribute to our overall goal
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will investigate the nuclear function of TPL-2 kinase. Techniques: Cell culture, protein-protein interaction studies, CRISPR/Cas9 gene editing, transcriptomic analysis by QPCR and RNA-seq. References: P
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) and their relationships with neighboring tissues in pathophysiological conditions. We utilize in vivo mouse genetics, live imaging, 3D organoids, genome-wide Cas9/Crispr based functional genetic
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-leading research in several fields. Notably, the groundbreaking discovery of the CRISPR-Cas9 gene-editing tool, which was awarded the Nobel Prize in Chemistry, was made here. At Umeå University, everything
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for hematological and solid cancers. Your position The successful candidate will have the opportunity to work with cutting-edge experimental methods, including CRISPR-Cas9, single-cell RNA and ATAC sequencing
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solid cancers. Your position The successful candidate will have the opportunity to work with cutting-edge experimental methods, including CRISPR-Cas9, single-cell RNA and ATAC sequencing, advanced high
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their relevance to ALS disease mechanisms. For more information about the project, or to discuss a potential application, please contact Dr Chun Guo (https://www.sheffield.ac.uk/biosciences/people
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. Cell culture and genetic manipulation, including lentivirus production, transduction, transfection, and CRISPR/Cas9 genome editing. 2. Live-cell imaging using confocal microscopy, along with quantitative
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CRISPR gene editing, flow cytometry, or next-generation sequencing. 3. Data Management: Proficiency with data management tools, statistical analysis software, and database systems like REDCap, Qualtrics