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, appropriate to an early career researcher. A4. Sufficient depth of CRISPR-cas9 based gene editing in primary cells, normally including sufficient postdoctoral experience in that field, appropriate to an early
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-cell suspensions and multi-parameter flow cytometry • Molecular cloning with focus on CRISPR-based approaches • Experience with viral vector systems (e.g., AAV production and purification) • Single-cell
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a thesis project in molecular or cellular biology. Experience in using CRISPR-based gene perturbation systems, endogenous-tagging of proteins using viral or non-viral systems or other equivalent
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ubiquitylation (http://www.ppu.mrc.ac.uk/). Many world-leading researchers in the field of signal transduction have trained within the MRC PPU. The major aims of the MRC PPU are to advance understanding
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-leading research in several fields. Notably, the groundbreaking discovery of the CRISPR-Cas9 gene-editing tool, which was awarded the Nobel Prize in Chemistry, was made here. At Umeå University, everything
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, or CRISPR/Cas9-based gene editing etc. The exact experimental platforms will be tailored to the applicant’s expertise and aligned with the project's needs. Working with human skin biopsies, wound tissue, and
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-leading research in several fields. Notably, the groundbreaking discovery of the CRISPR-Cas9 gene-editing tool, which was awarded the Nobel Prize in Chemistry, was made here. At Umeå University, everything
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, the IGI focuses on unraveling the mechanisms underlying CRISPR-based genome editing and applying this technology to cure disease, combat climate change ensure food sustainability, and provide additional
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liquid handling robots. Experience in bioimage analysis, for example using CellProfiler, R Bioconductor, ImageJ, and ML models. Experience in genetic (CRISPR) screening. Ability work efficiently and
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characterize novel photoreceptors in marine Synechococcus using CRISPR and protein biochemistry approaches. This project is funded by the NSF Systems and Synthetic Biology Program and builds on our successful