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localization-based measurements in cells. • Strong experience in human embryonic stem cell culture and CRISPR/Cas9-based genome engineering. • Demonstrated experience generating and validating genetically
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culture & mouse model animals Highly motivated, team-spirit oriented, well-developed collaborative skills & Mentoring skills Where to apply E-mail gaiddon@unistra.fr Requirements Research FieldBiological
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to identify metabolic alterations that drive tumor initiation and progression. This work has recently expanded to examine how metabolism and autophagy influence the biology of embryonic and cancer stem cells
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in-vitro research by running cell-based experiments that directly inform discovery decisions. Position Overview In this role, you will execute in-vitro experiments focused on cell culture, molecular
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-mediated diseases Assist with all aspects of experimental and administrative work with focus on cell culture and tissue histology experiments Isolate primary murine and human cells from peripheral blood
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human cell culture Knowledge of specialized methods for determining age-related parameters in the model organism Drosophila (e.g. memory, climbing, food consumption, etc.) (desirable) Didactic aptitude
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significance. Responsibilities Essential Functions 15%- Data analysis 20%- Imaging 10% - Immunohistochemistry 20% - Mouse treatment and experiment including perfusion 20%- Cell culture 15% - Laboratory
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(80%) Perform nerve histological analyses using immunofluorescence, RNAscope in situ hybridization, and ultrastructural approaches Conduct cell culture experiments with bone marrow?derived macrophages
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, qPCR, Western blotting, ELISA) are mandatory. Experience with stem cell isolation and differentiation/iPS cells is highly expected. Experience with culture of primary SMCs, cardiomyocytes, and/or
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and smooth muscle cells. For related publications, please see below: https://pubmed.ncbi.nlm.nih.gov/38898303/ https://pubmed.ncbi.nlm.nih.gov/31504245/ https://pubmed.ncbi.nlm.nih.gov/22896585