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; 3D cell culture; drug screening; Funding of the PhD candidate: GACR LA grant (GF24-10605K) & IP (0151) (PI: Lukas Trantirek) In the academic year 2026/27 the net income of the CEITEC PhD School student
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experience in cell culture Experience with in vivo experiments in mice (FELASA B certificate) is an advantage Strong planning and organizational skills, as well as ability to work independently English
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candidate is expected to: Design and perform advanced cell culture experiments on multiple cell lines Perform experiments to study cell behavior under different conditions Conduct multiparametric flow
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approaches at the interface between cell biology and mechanics, including advanced microscopy on living cells, cell biology (mammalian cell culture, transfection, molecular biology...) and micromanipulation
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data, immunology and cancer systems biology. Experience with genomic engineering methods, stem cell culture, flow cytometry, and murine models is preferred. Ability to work independently and
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includes developmental, computational, evolutionary and molecular biologists. References Elek, A. et al. Decoding cnidarian cell type gene regulation. bioRxiv 2025.07.01.662323 Preprint at https://doi.org
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regulation, combining single-cell genomics, chromatin profiling, and comparative genomics methods in a phylogenetically diverse array of eukaryotes. For more details about the lab, visit the lab website: https
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maintaining lab functions Perform mammalian cell culture, viral transduction, and molecular cloning Apply and develop methods in protein biochemistry Utilize advanced microscopy techniques, including confocal
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miRNAs in cell culture assay. Subsequently, novel small molecules targeting the identified miRNAs will then be generated and tested, first in cell culture and then in mouse models of accelerated aging
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culture and differentiation of human pluripotent stem cells (ESCs or iPSCs). · Strong experimental skills in molecular and cellular biology, including techniques such as RNA/protein expression analysis